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KMID : 0604520060320010017
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Journal of the Society of Cosmetic Scientists of Korea
2006 Volume.32 No. 1 p.17 ~ p.22
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Phosphatidylserine Enhances Skin Barrier Function Through Keratinocyte Differentiation
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Chung So-Young
Nam Sang-Joon
Choi Wang-Keun
Seo Mi-Young
Kim Jin-Wook
Lee Seung-Hun
Park Chang-Seo
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Abstract
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Phosphatidyiserine (PS) is a phospholipid which plays the structural role in membranes and serves as a cofactor of signaling enzymes for diverse cellular functions. In this study, we observed that topical treatment with PS significantly decreased trans-epidermal water loss (TEWL) induced by tape-stripping in hairless mice. Also, ceramides in epidermis were increased in PS-treated group compared to vehicle-treated one in vivo. the amounts of non-hydroxyl ceramide (NHCER) (1.4 fold) and glucosylceramide (glucosylCER) (1.6 fold), in the skin of hairless mice, were increased by topical treament with PS. Also, we demonstrated that PS stimulated keratinocyte differentiation. We observed that PS treatment morphologically altered normal human keratinocyte (NHK) from the proliferating phase to the differentiating one, suggesting that PS stimulated epidermal differentiation in NHK. We also showed that the expressions of the specific markers for epidermal differentiation, involucrin (INV) (3.5 fold up) and transglutaminase 1 (TG’’ase 1) (3 fold up), were significantly increased by PS treatment, compared to untreated control in vitro. In addition, topical treatment with PS resulted in a progressive increase in INV and loricrin protein levels in vivo. In conclusion, we provide the first evidence for the physiological activities of PS in skin, and we suggest that PS strengthen the epidermal permeability harrier by stimulation of keratinocyte differentiation.
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KEYWORD
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Phosphatidylserine, keratinocyte differentiation, ceramide, TEWL
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